The TMB substrate reagent set is designed for use in BD OptEIA™ ELISA sets. Substrate Reagent A, which is clear and colorless, contains hydrogen peroxide. Substrate Reagent B is a colorless to a very light amber solution containing 3,3', 5,5' tetramethylbenzidine (TMB) in an organic solvent.
Preparation and storage
Store unopened reagents at 2-8°C. Do not use after the expiration date. Discard any remaining working solutions after use. Reagents should be brought to room temperature (20-25°C) prior to use. Allow at least 30 minutes for this process. Avoid prolonged exposure to light, contact with metal, air or extreme temperatures as color may develop.
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